Journal article
Empirically derived formulae for calculation of age- and region-related levels of iron, copper and zinc in the adult C57BL/6 mouse brain
E Suryana, BD Rowlands, DP Bishop, DI Finkelstein, KL Double
Neurobiology of Aging | ELSEVIER SCIENCE INC | Published : 2024
Abstract
Metal dyshomeostasis is associated with neurodegenerative disorders, cancers and vascular disease. We report the effects of age (range: 3 to 18 months) on regional copper, iron and zinc levels in the brain of the C57BL/6 mouse, a widely used inbred strain with a permissive background allowing maximal expression of mutations in models that recapitulate these disorders. We present formulae that can be used to determine regional brain metal concentrations in the C57BL/6 mouse at any age in the range of three to eighteen months of life. Copper levels in the C57BL/6 mouse adult brain were highest in the striatum and cerebellum and increased with age, excepting the cortex and hippocampus. Regional..
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Awarded by Parkinson's NSW
Funding Acknowledgements
This study was supported by funding by Parkinson's NSW and the National Health and Medical Research Council of Australia (NHMRC) Ideas grant 1181864. We thank A/Prof Dominic Hare for his assistance in the preparation of samples for ICP-MS. We thank Dr Jia Luo (University of Sydney) for lyophilising the tissue samples. We also wish to acknowledge the support of Lydia Gunawan and Amelia Sedjahtera (The Florey Institute of Neuroscience and Mental Health) for their care of the animals.